ワタナベ ナカミチ    WATANABE Nakamichi
   渡辺 睦行
   所属
食健康科学部 健康デザイン学科
 
生活機構研究科 生活科学研究専攻
 
女性健康科学研究所 所属教員
   職種
准教授
言語種別 英語
発行・発表の年月 2015
形態種別 学術雑誌
査読 査読あり
標題 Fatigue alleviation mechanism of citric acid determined by gene expression analysis in the mouse liver
執筆形態 共著
掲載誌名 Food and Nutrition Sciences
掲載区分国外
巻・号・頁 6(12),pp.1095-1102
著者・共著者 Yurie Hara, Nakamichi Watanabe
概要 Citric acid has been recognized for its ability to alleviate fatigue; however, the mechanism contri- buting to this effect has not yet been elucidated. Our previous study suggested that the alleviation of fatigue by citric acid intake might be induced by the acceleration of gluconeogenesis. In this study, we tested this hypothesis and evaluated whether the intake of citric acid accelerates gluco- neogenesis in the mouse liver through microarray analysis. Because the microarray of focus in- cludes 200 metabolic genes, it could be used to provide information on genes related to glucose metabolism as well as other pathways such as inflammation. Eight-week-old male C57/BL6J mice were divided into the distilled water and citric acid groups (n = 6), and were administered distill- ed water or a citric acid solution (20 mg/30g body weight) intragastrically with a probe, respec- tively. Forty minutes after administration, the mice were sacrificed by decapitation. The blood glucose level of the citric acid group was significantly higher (P = 0.001) than that of the distilled water group. The expression levels of genes related to glucose metabolism (Gck, Pfkl, Pklr, Pck1, G6pc) were not changed by the intake of citric acid. On the other hand, some transcriptional dif- ferences were observed in several other genes, including those related to inflammation, blood pressure, the electron transport system, uncoupling proteins, and anti-oxidation. The expression levels of genes related to inflammation were reduced in the citric acid group; e.g., the Il6 expres- sion level was 0.78 times lower in the citric acid group than that in the distilled water group. In conclusion, the results of this study suggest that citric acid intake could accelerate gluconeogene- sis and suppresses inflammation. Therefore, the fatigue-alleviation effects of citric acid might be induced by an additive effect between the maintenance of blood glucose levels and the suppres- sion of inflammation.