ハヤシ マリコ    Hayashi Mariko
   林 真理子
   所属
食健康科学部 管理栄養学科
 
生活機構研究科 生活科学研究専攻
 
女性健康科学研究所 所属教員
   職種
准教授
言語種別 英語
発行・発表の年月 2015/03
形態種別 学術雑誌
査読 査読あり
標題 The transmembrane transporter domain of glutamate transporters is a process tip localizer
執筆形態 共著
掲載誌名 Sci Rep
掲載区分国外
巻・号・頁 5,pp.9032
著者・共著者 ◎Hayashi MK, Yasui M
概要 Glutamate transporters in the central nervous system remove glutamate released from neurons to terminate the signal. These transporters localize to astrocyte process tips approaching neuronal synapses. The mechanisms underlying the localization of glutamate transporters to these processes, however, are not known. In this study, we demonstrate that the trimeric transmembrane transporter domain fragment of glutamate transporters, lacking both N- and C-terminal cytoplasmic regions, localized to filopodia tips. This is a common property of trimeric transporters including a neutral amino acid transporter ASCT1. Astrocyte specific proteins are not required for the filopodia tip localization. An extracellular loop at the centre of the 4th transmembrane helices, unique for metazoans, is required for the localization. Moreover, a C186S mutation at the 4th transmembrane region of EAAT1, found in episodic ataxia patients, significantly decreased its process tip localization. The transmembrane transporter domain fragments of glutamate transporters also localized to astrocyte process tips in cultured hippocampal slice. These results indicate that the transmembrane transporter domain of glutamate transporters have an additional function as a sorting signal to process tips.
researchmap用URL https://www.nature.com/articles/srep09032