コンドウ　カズナリ    KONDO KAZUNARI
   近藤 一成
   所属
食健康科学部 食安全マネジメント学科
   職種
教授
言語種別 英語
発行・発表の年月 2013
形態種別 学術雑誌
査読 査読あり
標題 Development and validation of event-specific quantitative PCR method for genetically modified maize LY038.
執筆形態 共著
掲載誌名 Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi)
掲載区分国内
巻・号・頁 54,pp.25-30
著者・共著者 Mano J, Masubuchi T, Hatano S, Futo S, Koiwa T, Minegishi Y, Noguchi A, Kondo K, Akiyama H, Teshima R.
概要 In this article, we report a novel real-time PCR-based analytical method for quantitation of the GM maize event LY038. We designed LY038-specific and maize endogenous reference DNA-specific PCR amplifications. After confirming the specificity and linearity of the LY038-specific PCR amplification, we determined the conversion factor required to calculate the weight-based content of GM organism (GMO) in a multilaboratory evaluation. Finally, in order to validate the developed method, an interlaboratory collaborative trial according to the internationally harmonized guidelines was performed with blind DNA samples containing LY038 at the mixing levels of 0, 0.5, 1.0, 5.0 and 10.0%. The precision of the method was evaluated as the RSD of reproducibility (RSDR), and the values obtained were all less than 25%. The limit of quantitation of the method was judged to be 0.5% based on the definition of ISO 24276 guideline. The results from the collaborative trial suggested that the developed quantitative method would be suitable for practical testing of LY038 maize.