ワタナベ ナカミチ    WATANABE Nakamichi
   渡辺 睦行
   所属
食健康科学部 健康デザイン学科
 
生活機構研究科 生活科学研究専攻
 
女性健康科学研究所 所属教員
   職種
准教授
言語種別 英語
発行・発表の年月 2021
形態種別 学術雑誌
査読 査読あり
標題 Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats
執筆形態 共著
掲載誌名 Heliyon
掲載区分国外
出版社・発行元 Cell Press
巻・号・頁 7,pp.e08501
著者・共著者 Yurie Hara, Satoshi Kume, Yosky Kataoka, Nakamichi Watanabe
概要 Recent studies have reported that plasma levels of tricarboxylic acid (TCA) cycle metabolites and TCA cycle- related metabolite change in patients with chronic fatigue syndrome (CFS) and in healthy humans after exer- cise. Exogenous dietary citric acid has been reported to alleviate fatigue during daily activities and after exercise. However, it is unknown whether dietary citric acid affects the plasma levels of these metabolites. Therefore, the present study aimed to investigate the effects of exogenously administered citric acid on TCA cycle metabolites and TCA cycle-related metabolites in plasma. Sprague-Dawley rats were divided into control and citric acid groups. We evaluated the effect of exogenous dietary citric acid on the plasma TCA cycle and TCA cycle-related metabolites by metabolome analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS). TCA cycle metabolites, including plasma citrate, cis-aconitate, and isocitrate, were significantly elevated after exog- enous administration of citric acid. Anaplerotic amino acids, which are converted to TCA cycle metabolites, such as serine, glycine, tryptophan, lysine, leucine, histidine, glutamine, arginine, isoleucine, methionine, valine, and phenylalanine, also showed significantly elevated levels. Citric acid administration significantly increased the levels of initial TCA cycle metabolites in the plasma. This increase after administration of citric acid was shown to be opposite to the metabolic changes observed in patients with CFS. These results contribute novel insight into the fatigue alleviation mechanism of citric acid.