ムラマツ　シュキ    MURAMATSU Shuki
   村松　朱喜
   所属
食健康科学部 健康デザイン学科
 
女性健康科学研究所 所属教員
 
国際文化研究所 所属教員
 
生活機構研究科 生活科学研究専攻
   職種
准教授
言語種別 英語
発行・発表の年月 2007/01
形態種別 学術雑誌
査読 査読あり
標題 Isolation and transcriptional regulation of the fructose 1,6-bisphosphate aldolase gene FBA1 in the psychrophilicyeast Cystofilobasidium capitatum.
執筆形態 共著
掲載誌名 Food Preservation Science
掲載区分国外
出版社・発行元 Japan Association of Food Preservation Science
巻・号・頁 33(1),pp.9-13
著者・共著者 ◎Fujimura, S., Uchino, M., Ito, T., Myoda, T., Nagaoka T., Miyaji, T., Nakagawa T., Takano K. and Tomizuka, N.
概要 In this study, we report the primary structure of fructose 1,6-bisphosphate aldolase encoded by Cystofilobasidium capitatum FBA1 (CcFBA1) and the regulation of its gene expression. CcFBA1 consists of a 1,080 bp ORF corresponding to a protein of 360 amino acid residues, and the calculated molecular weight of CcFba1p is 39,626 Da. The CcFba1p has both aldolase class-II signatures 1 and 2. CcFBA1 is expressed at the same level during growth on several different carbon sources, although expression level may be slightly higher on glucose. Transcription of CcFBA1 is not influenced by temperature or existence of oxygen, although it was reduced during the stationary phase. We show that CcFba1p is a constitutive enzyme in C. capitatum, and suggest that FBA1 could be used as a marker for the detection of pectinolytic psychrophilic yeasts using the PCR method.