ムラマツ シュキ    MURAMATSU Shuki
   村松 朱喜
   所属
食健康科学部 健康デザイン学科
 
女性健康科学研究所 所属教員
   職種
専任講師
言語種別 英語
発行・発表の年月 2020/09
形態種別 学術雑誌
査読 査読あり
標題 Isolation and characterization of a motility-defective mutant of Euglena gracilis.
執筆形態 共著
掲載誌名 PeerJ
掲載区分国外
巻・号・頁 10.7717/peerj.10002. eCollection (2020)
著者・共著者 Muramatsu S., Atsuji K., Yamada K., Ozasa K., Suzuki H., Takeuchi T., Hashimoto-Marukawa Y., Kazama Y., Abe T., Suzuki K., Iwata O
概要 Euglena gracilis is a green photosynthetic microalga that swims using its flagellum. This species has been used as a model organism for over half a century to study its metabolism and the mechanisms of its behavior. The development of mass-cultivation technology has led to E. gracilis application as a feedstock in various products such as foods. Therefore, breeding of E. gracilis has been attempted to improve the productivity of this feedstock for potential industrial applications. For this purpose, a characteristic that preserves the microalgal energy e.g., reduces motility, should be added to the cultivars. The objective of this study was to verify our hypothesis that E. gracilis locomotion-defective mutants are suitable for industrial applications because they save the energy required for locomotion. To test this hypothesis, we screened for E. gracilis mutants from Fe-ion-irradiated cell suspensions and established a mutant strain,
M3ZFeL, which shows defects in flagellum formation and locomotion. The mutant strain exhibits a growth rate comparable to that of the wild type when cultured under autotrophic conditions, but had a slightly slower growth under heterotrophic conditions. It also stores 1.6 times the amount of paramylon, a crystal of β-1,3-glucan, under autotrophic culture conditions, and shows a faster sedimentation compared with that of the wild type, because of the deficiency in mobility and probably the high amount of paramylon accumulation. Such characteristics make E. gracilis mutant cells suitable for cost-effective mass cultivation and harvesting.