ムラマツ シュキ    MURAMATSU Shuki
   村松 朱喜
   所属
食健康科学部 健康デザイン学科
 
女性健康科学研究所 所属教員
   職種
専任講師
言語種別 英語
発行・発表の年月 2010/10
形態種別 学術雑誌
査読 査読あり
標題 Transcription factor Stb5p is essential for acetoaldehyde tolerance in Saccharomyces cerevisiae.
執筆形態 共著
掲載誌名 Journal of Basic Microbiology
掲載区分国外
出版社・発行元 John Wiley & Sons, Inc.
巻・号・頁 50(5),pp.494-498
著者・共著者 ◎Matsufuji, Y., Nakagawa, T., Fujimura, S., Tani, A. and Nakagawa, J.
概要 Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, Δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.