ムラマツ シュキ    MURAMATSU Shuki
   村松 朱喜
   所属
食健康科学部 健康デザイン学科
 
女性健康科学研究所 所属教員
   職種
専任講師
言語種別 英語
発行・発表の年月 2010/08
形態種別 学術雑誌
査読 査読あり
標題 The peroxisomal catalase gene in the methylotrophic yeast Pichia methanolica.
執筆形態 共著
掲載誌名 Bioscience, Biotechnology, and Biochemistry
掲載区分国外
出版社・発行元 Society for Bioscience, Biotechnology, and Agrochemistry
巻・号・頁 74(8),pp.1733-1735
著者・共著者 ◎Nakagawa, T., Yoshida, K., Takeuchi, A., Ito, T., Fujimura, S., Matsufuji, Y., Tomizuka, N., Yurimoto, H., Sakai, Y. and Hayakawa T.
概要 In this paper, we describe the CTA1 gene, which encodes a peroxisomal catalase in the methylotrophic yeast Pichia methanolica. The P. methanolica CTA1 gene (PmCTA1) comprises a 1,530-bp open reading frame corresponding to a protein of 510 amino acid residues, and its deduced amino acid sequence shows high similarity to those of Cta1ps from other methylotrophic yeasts (about 79%). Expression of PmCTA1 in a peroxisomal catalase-depleted (Cbcta1Delta) Candida boidinii strain restored the methylotrophic growth of the host strain, while the expression of PmCTA1-DeltaSRL, which lacks peroxisome targeting signal type 1, did not. In P. methanolica, expression of PmCTA1 was induced when cells were grown on peroxisome-inducing carbon sources, viz., methanol, oleate, and D-alanine. Taken together, these results indicate that PmCTA1 encodes a functional peroxisomal catalase in P. methanolica.