ムラマツ シュキ    MURAMATSU Shuki
   村松 朱喜
   所属
食健康科学部 健康デザイン学科
 
女性健康科学研究所 所属教員
   職種
専任講師
言語種別 英語
発行・発表の年月 2010/07
形態種別 学術雑誌
査読 査読あり
標題 Molecular characterization of two genes with high similarity to the dihydroxyacetone synthase gene in the methylotrophic yeast Pichia methanolica.
執筆形態 共著
掲載誌名 Bioscience, Biotechnology,and Biochemistry
掲載区分国外
出版社・発行元 Society for Bioscience, Biotechnology, and Agrochemistry
巻・号・頁 74(7),pp.1491-1493
著者・共著者 Nakagawa, T., Fujimura, S., Ito, T., Matsufuji, Y., Ozawa, S., Miyaji, T., Nakagawa, J., Tomizuka, N., Yurimoto, H., Sakai, Y. and Hayakawa, T.
概要 The methylotrophic yeast Pichia methanolica possesses two genes, PmDAS1 and PmDLP1, whose amino acid sequences show high similarity to dihydroxyacetone synthase (DAS), the formaldehyde-fixing enzyme for methanol metabolism within the peroxisome. The PmDAS1 and PmDLP1 genes encode 709 and 707 amino acid residues respectively, and PmDas1p contains a type-1 peroxisomal targeting signal (PTS1), while PmDlp1p does not. Upon phylogenetic analysis, PmDas1p fit into the DAS group with other DASs, while PmDlp1p was grouped with the DAS-like proteins (DLP) of non-methylotrophic yeasts and fungi, a branch of the phylogenetic tree independent of the DAS and transketolase (TK) groups. While expression of PmDAS1 restored the methylotrophic growth of the Candida boidinii das1Delta strain, the PmDLP1 and PmDAS1-DeltaPTS1 genes did not. Taken together, these results indicate that PmDAS1 encodes a functional DAS and has an indispensable role in methanol metabolism, and that PmDlp1p share a common, as yet uncharacterized function in P. methanolica as well as in non-methylotrophic yeasts and fungi.