ウメガキ ケイゾウ    UMEGAKI Keizo
   梅垣 敬三
   所属
食健康科学部 食安全マネジメント学科
 
生活機構研究科 生活科学研究専攻
 
研究支援機器センター 所属教員
 
生活機構研究科 生活機構学専攻
   職種
教授
言語種別 英語
発行・発表の年月 2019/11
形態種別 学術雑誌
査読 査読あり
標題 Tandem Cation/Anion Exchange SPE Cartridge Method for Sample Desalting for HPLC Analysis of Soluble Dietary Fiber: Development and Inter-laboratory Validation.
執筆形態 共著
掲載誌名 Anal Sci
掲載区分国外
巻・号・頁 35(11),pp.1269-1272
著者・共著者 Suzuki I, Kumai Y, Kitagawa M, Kishimoto Y, Umegaki K, Chiba T, Takebayashi J.
概要 In HPLC analyses of soluble dietary fiber, desalting processes using open, mixed-bed ion-exchange columns are time-consuming and labor-intensive. We developed and validated a simple desalting method using tandem cation/anion exchange SPE cartridges. We found that combining Bond Elut Jr SCX (upstream) and Bond Elut PSA (downstream) cartridges provided adequate desalting of test solutions. The developed method was then validated in an inter-laboratory study. Five test samples were prepared by mixing food matrixes with purified soluble dietary fiber and treated to generate solutions to test the desalting process. These solutions were then analyzed by eight different laboratories. The results demonstrated that the developed method is simple and reliable for desalting samples containing 140 to 945 mg/100 mL of soluble dietary fiber in preparation for HPLC analysis of soluble dietary fiber.