ウメガキ ケイゾウ    UMEGAKI Keizo
   梅垣 敬三
   所属
食健康科学部 食安全マネジメント学科
 
生活機構研究科 生活科学研究専攻
 
研究支援機器センター 所属教員
 
生活機構研究科 生活機構学専攻
   職種
教授
言語種別 英語
発行・発表の年月 2017
形態種別 学術雑誌
査読 査読あり
標題 Evaluation of plasma antioxidant activity in rats given excess EGCg with reference to endogenous antioxidants concentrations and assay methods.
執筆形態 共著
掲載誌名 Free Radic Res
掲載区分国外
巻・号・頁 51(2),pp.193-199
著者・共著者 Yokotani, K. Umegaki K
概要 We examined how administration of EGCg contributes to plasma antioxidant activity, relative to its concentration, endogenous antioxidants, and assay methods, namely oxygen radical absorbance capacity (ORAC) and ferric reducing/antioxidant power (FRAP). Administration of EGCg (500 mg/kg) to rats increased plasma EGCg (4mumol/L as free form) and ascorbic acid (1.7-fold), as well as ORAC (1.2-fold) and FRAP (3-fold) values. The increase in plasma ascorbic acid following EGCg administration was accompanied by its relocation from the adrenal glands and lymphocytes into plasma, and was related to the increase in FRAP. Plasma deproteinization and assays in plasma model solutions revealed that protein levels significantly contributed to ORAC values, where <3 mumol/L EGCg in the presence of protein exhibited minimal antioxidant activity, as measured by both FRAP and ORAC. As the concentration of plasma ascorbic acid was not influenced by deproteinization, differences in FRAP values with and without deproteinization were estimated to determine the contribution of enhanced ascorbic acid attributable to EGCg administration.