カワサキ ヒロアキ
KAWASAKI Hiroaki
川崎 広明 所属
食健康科学部 管理栄養学科
女性健康科学研究所 所属教員
職種
専任講師
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発表年月日 | 2010/06 |
発表テーマ | Generation of 6-nitrotryptophan residues in the proteins of PC12 cell lysate by a peroxynitrite treatment. |
発表学会名 | The 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide (NO2010) |
学会区分 | 国際学会 |
発表形式 | ポスター |
単独共同区分 | 共同 |
開催地名 | Kyoto |
発表者・共同発表者 | Kawasaki H, Ikeda K, Shigenaga A, Baba T, Ogawa H, Takamori K, Yamakura F. |
概要 | We constructed the methods to identified nitrated tryptophan containing protein from PC12 cell lysates exposed to peroxynitrite. Proteomic analysis using the monoclonal and polyclonal antibodies against nitrated tryptophan and LC-MS/MS were important techniques in this methods. Using this method, we achieved to detect some nitrated tryptophan containing proteins which played critical roles of glycolysis. |