カワサキ ヒロアキ
KAWASAKI Hiroaki
川崎 広明 所属
食健康科学部 管理栄養学科
女性健康科学研究所 所属教員
職種
准教授
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発表年月日 | 2009/11 |
発表テーマ | Identification of nitrotryptophan by the LC-ESI-MS/MS analysis in the nitrated proteins generated by the treatment of PC12 cell lysate with peroxynitrite. |
発表学会名 | SFRBM 2009-16th Annual Meeting of the Society for Free Radical Biology and Medicine |
学会区分 | 国際学会 |
発表形式 | ポスター |
単独共同区分 | 共同 |
開催地名 | San Francisco |
発表者・共同発表者 | Kawasaki H, Ikeda K, Taka H, Fujimura T, Baba T, Ogawa H, Takamori K, Yamakura F. |
概要 | We constructed the methods to identified nitrated tryptophan containing protein from PC12 cell lysates exposed to peroxynitrite. Proteomic analysis using the monoclonal and polyclonal antibodies against nitrated tryptophan and LC-MS/MS were important techniques in this methods. Using this method, we achieved to detect some nitrated tryptophan containing enzymes in the cell lysates. |