カワサキ ヒロアキ    KAWASAKI Hiroaki
   川崎 広明
   所属
生活科学部 管理栄養学科
   職種
専任講師
発表年月日 2009/11
発表テーマ Identification of nitrotryptophan by the LC-ESI-MS/MS analysis in the nitrated proteins generated by the treatment of PC12 cell lysate with peroxynitrite.
発表学会名 SFRBM 2009-16th Annual Meeting of the Society for Free Radical Biology and Medicine
学会区分 国際学会
発表形式 ポスター
単独共同区分 共同
開催地名 San Francisco
発表者・共同発表者 Kawasaki H, Ikeda K, Taka H, Fujimura T, Baba T, Ogawa H, Takamori K, Yamakura F.
概要 We constructed the methods to identified nitrated tryptophan containing protein from PC12 cell lysates exposed to peroxynitrite. Proteomic analysis using the monoclonal and polyclonal antibodies against nitrated tryptophan and LC-MS/MS were important techniques in this methods. Using this method, we achieved to detect some nitrated tryptophan containing enzymes in the cell lysates.